Debutant Jonny Smith scored a last-minute winner as below-par Hull City slumped to a disappointing defeat against bottom club Burton Albion.
The Tigers lacked their usual attacking threat, Greg Docherty going closest when he crashed an effort against the bar.
Josh Parker spurned a golden opportunity for the Brewers but provided the assist for substitute Smith to steer home in the final minute of the 90.
Captain Richie Smallwood replaced the injured Alfie Jones in the only change from the last league game against Swindon Town.
Hull City stormed to a 5-0 win on their only previous visit to the Pirelli Stadium in April 2018 but the latest meeting proved a far more competitive affair.
Callum Elder, with six assists this season, looked to add another to his name on 10 minutes with a pinpoint left-wing delivery which Keane Lewis-Potter headed marginally over.
There was a warning for the Tigers moments later as Josh Earl burst past Lewie Coyle and crossed low into the box.
A sliding Jacob Greaves cut the cross out ahead of Kane Hemmings, with the loose ball falling for Lucas Akins to scuff a left-footed effort into the side-netting.
City were forced into a change on 22 minutes as George Long collided with Coyle and Smallwood when coming for a free-kick, the latter unable to continue and replaced by Regan Slater.
Despite the 32-point gap between the teams, the Tigers were certainly not having things their own way.
With half-time approaching, Dan Crowley manufactured some space before curling towards the far top corner, but his aim was slightly off.
The deadlock remained unbroken heading into the second half; Sean Clare fired harmlessly wide from distance and Greg Docherty had similar luck with a long-range effort.
The Scot went much closer with his next attempt on 57 minutes, striking a sweet left-footed shot against the top of the bar from 20 yards.
Long had been a virtual spectator but had to be alert to parry Smith’s driven strike from an acute angle after 70 minutes – the first shot on target.
Crowley then curled wide in a carbon copy of his first-half attempt – his final contribution before being withdrawn for Josh Magennis.
James Scott was next to enter the fray as he replaced Mallik Wilks - moments after Jimmy Floyd Hasselbaink’s side should have taken the lead.
Tom Hamer’s low cross was palmed by Long into the path of Josh Parker but, with Coyle lunging towards him, the forward side-footed wide from close range.
The Brewers were growing in belief and Joe Powell was inches away from finding the top corner with a 25-yard free-kick which curled agonisingly wide.
Albion had looked the more threatening in the latter stages and struck the killer blow in the final minute of normal time.
A clipped ball from Parker was taken down by Smith, who held off the challenge of Elder before steering low past Long, leaving the Tigers defeated and deflated.
Burton Albion: Ben Garratt, John Brayford ©, Ryan Edwards (Terry Taylor 74’), Kane Hemmings (Joe Powell 45’), Lucas Akins, Josh Parker, Josh Earl, Hayden Carter, Ryan Broom (Jonny Smith 63’), Sean Clare, Tom Hamer.
Subs not used: Dillon Barnes, Kieran Wallace, Owen Gallacher, Ben Hart.
Hull City: George Long; Lewie Coyle, Reece Burke, Jacob Greaves, Callum Elder; Richie Smallwood © (Regan Slater 22’), George Honeyman, Greg Docherty; Dan Crowley (Josh Magennis 71’), Keane Lewis-Potter, Mallik Wilks (James Scott 77’).
Subs not used: Matt Ingram, Josh Emmanuel, Jordan Flores, Max Clark.